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Image Search Results
Journal: Cell Research
Article Title: Driving vascular endothelial cell fate of human multipotent Isl1 + heart progenitors with VEGF modified mRNA
doi: 10.1038/cr.2013.112
Figure Lengend Snippet: Endothelial differentiation of the human Isl1 + progenitors can be achieved by repeated transfections of the VEGF-A modRNA. (A) FACS result showing expression of mCherry in the day-7 purified human Isl1-cre eGFP + cells 24 h post transfection of the mCherry modRNA (1 μg/10 6 cells). (B) Quantification of mCherry expression by FACS showing percentage of mCherry + Isl1-cre eGFP + cells following 0-150 h post transfection. (C) ELISA analyses using VEGF-A-containing supernatant (refreshed 6 h before collection) cultured with the day-7 purified human Isl1-cre eGFP + cells following 0-72 h post transfection of different concentrations of the VEGF-A modRNA. (D) Trypan blue staining showing the survival percentage of the day-7 purified human Isl1-cre eGFP + cells following 24 h post transfection of different concentrations of the VEGF-A modRNA. (E) ELISA analyses showing the rate of VEGF-A secreted by the day-7 purified human Isl1-cre eGFP + cells (supernatant refreshed 6 h before collection) following two rounds of daily transfections of 1 μg/10 6 cells with VEGF-A modRNA. (F) FACS analyses showing the efficiency of CD144 + CD31 + EC differentiation by one transfection or repeated transfections (following medium change) of the VEGF-A modRNA compared to the use of VEGF-A protein 3 or 7 days post treatment.
Article Snippet: In vivo transfection was performed by adding the
Techniques: Transfection, Expressing, Purification, Enzyme-linked Immunosorbent Assay, Cell Culture, Staining